Data to support "Proline-conditioning and chemically-programmed ice nucleation protects spheroids during cryopreservation"

Cryopreserving spheroids, however, remains challenging as conventional cryoprotectants do not mitigate all damage mechanisms. Here chemically-programmed extracellular ice nucleation is used to prevent supercooling, alongside proline pre-conditioning, which are found to synergystically improve post-thaw recovery of spheroids.

This validates the need to identify compounds and materials to address both biochemical and biophysical damage pathways beyond standard cryoprotectants.<br><br>Dataset file to support the article published in Chemical Communications