Data · dataset · 2026
Analysis of a novel antimicrobial peptide QUB-1746 from the skin secretion of the frog, Kassina senegalensis
Listed in UCL Research Data Repository
It is well-known that the skin secretions of amphibians contain abundant bioactive compounds, such as biogenic amines, steroids, alkaloids, peptides and proteins.
Description
In this thesis, the full-length cDNA sequence of a novel antimicrobial peptide, named QUB-1746, was cloned from a Kassina senegalensis skin secretion derived cDNA library through ‘shotgun’ cloning. The mature peptide was synthesized by solid phase peptide synthesis (SPPS).
RP-HPLC and MALDI-TOF MS were used to purify and analyze the mature peptide QUB-1746. Its minimal inhibitory concentration (MIC) was detected by using different micro-organisms and its haemolytic activity was tested by using horse red blood cells. MTT assay tested two human lung cancer cell lines, H23 and H157, for effects.
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The results of antimicrobial experiments showed that QUB-1746 was active only against the Gram-positive bacterium, S.aureus with an MIC value of 32 μM. The results of MTT assays showed that QUB-1746 had inhibitory activity against human lung cancer cells, H23 with an IC50 of 40.3 μM. Also, QUB-1746 possessed little haemolytic activity at less than 20% at 512 μM.<br><br><i>Thesis embargoed until 31st October 2024 </i>
Links
Where it is published
- DOI doi.org/10.17034/32628264.v1 ↗
DOI / persistent id · from rdr ucl ac uk
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from rdr ucl ac uk
Topics
- From keywords
- Earth & Environmental Science
- Inferred from text
- Cancer 75%
Provenance · 1 source records, 6 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| UCL Research Data Repository | oai:figshare.com:article/32628264 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[disease].local:disease:cancer | enrichment · rdr ucl ac uk | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| description | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/description |
| license_text | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| publication_date | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| title | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/title |