Data · dataset · 2026
<p>List of plasmids used in this study.</p>
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.1371/journal.pone.0349465.s008
<div><p>The origin recognition complex (ORC) binds origins of replication and directs the loading of the Mcm2–7 replicative helicase at these sites.
Description
Five of the six ORC subunits are related to the AAA+ family of ATPases. Although functions for ATP hydrolysis by Cdc6 and the Mcm2–7 complex have been described, the essential role of ORC ATP hydrolysis remains unclear.
We performed a genetic screen in <i>Saccharomyces cerevisiae</i> for suppressors of the lethal phenotype of the <i>orc4-R267A</i> allele, which disrupts ORC ATP hydrolysis <i>in vitro</i>. We identified six causative mutations, five of which are distributed across different ORC subunits. The suppressor mutations in Orc1 and Orc4, but not those in other ORC subunits, increase the <i>in vitro</i> helicase loading activity of ATPase-defective ORC (ORC4R).
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Allele specificity studies show the alleles specifically suppress defects at ATPase interfaces within the ORC-Cdc6 complex. The sixth allele is a mutation in <i>TOA2</i>, a subunit of the TFIIA general transcription factor. Mutations in the general transcription factors TBP and TFIIB, and the large subunit of RNA Polymerase II also suppress <i>orc4-R267A</i> lethality, suggesting that reducing transcription is sufficient for suppression<i>.</i> Our study identifies multiple pathways to suppress the lethal phenotype of an ATPase-defective ORC allele and reveals a connection between ORC ATP hydrolysis and transcription.</p></div>
Links
Where it is published
- DOI doi.org/10.1371/journal.pone.0349465.s008 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Biochemistry and cell biology · Biochemistry and cell biology · Biochemistry and cell biology · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Genetics · Genetics · Genetics · Life Sciences · Life Sciences · Life Sciences · Saccharomyces cerevisiae · Saccharomyces cerevisiae · Saccharomyces cerevisiae
Provenance · 3 source records, 20 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/34035564 | 12 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/34035564 | 12 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/34035564 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].anzsrc:group:3101 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3101 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3101 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3105 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3105 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3105 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[organism].NCBITaxon:4932 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['saccharomyces cerevisiae </'] |
| concepts[organism].NCBITaxon:4932 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['saccharomyces cerevisiae </'] |
| concepts[organism].NCBITaxon:4932 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['saccharomyces cerevisiae </'] |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/rights |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |