Immunoinformatics approach to design chimeric CD70-Peptide Vaccines against Renal cell carcinoma

Hence, the aim of the current investigation is to create a multi-epitope vaccine targeting overexpressed CD70 using an immunoinformatics technique. Herein, multi-epitope vaccines were constructed in silico by linking specific epitopes of CD70 protein for helper T lymphocytes and CD8+ T lymphocytes.

In order to boost its immunogenicity, sequences of cell penetrating peptide (CPP), penetratin (pAntp), as well as TNF-α whole sequence were bound to the N-terminal and C-terminal of the CD70 epitopes. Chimeric vaccines were assessed computationally in terms of antigenicity, allergenicity, peptide toxicity, population coverage and physicochemical properties. Moreover, various analyses were additionally conducted on refined 3D constructs, comprising of structural B–cell epitope prediction, molecular docking, molecular dynamics simulation, immune simulation analysis and in silico cloning of the vaccine constructs.