Excel · study · 2026
Single-cell RNA analysis of allele-specific MHC expression in macaque PBMC after T cell activation or treatment with pro-inflammatory or anti-inflammatory cytokines
Listed in NCBI GEO
We have employed a single-cell sequencing approach using 10X Genomics 5'scRNAseq to study expression of the Major Histocompatibility Complex (MHC) in peripheral blood mononuclear cells (PBMC) from Mauritian-origin cynomolgus and Indian-origin rhesus macaques.
Description
The MHC region has undergone a complex series of duplications in macaques relative to humans. Steady-state RNA levels of macaque MHC class I and class II transcripts vary over several orders of magnitude in whole blood and bulk PBMC.
In this study, we compared transcript levels in untreated control PBMC with transcript levels after T cell activation, treatment with pro-inflammatory cytokines, or treatment with anti-inflammatory cytokines. Transcript levels for several minor MHC alleles were preferentially induced in monocytes and dendritic cells after treatment with pro-inflammatory cytokines or T cell activation.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE329nnn/GSE329692 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE329692 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1436580 ncbi.nlm.nih.gov/bioproject/PRJNA1436580 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Macaca mulatta · Other
- From keywords
- Life Sciences
- Inferred from text
- Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE329692 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9544 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |