<b>Historical Experimental Records, Quantitative Analysis, and Author-Prepared Figure Reconstruction</b>

Retrospective examination of the historical source material verified the sample and lane identities, protein-specific measurements, corresponding loading controls, and quantitative calculations.</p><p dir="ltr">The author-prepared reconstruction documents the identified presentation error in the published tubulin panel and replaces the affected image using original tubulin source material. It is provided for independent scientific examination and <b>is not a formally published correction to the article</b>.</p><p dir="ltr"><b>2.

Repository Organization</b></p><table><tr><td><p dir="ltr"><b>Folder</b></p></td><td><p dir="ltr"><b>Contents</b></p></td></tr><tr><td><p dir="ltr">00_README</p></td><td><p dir="ltr">This README</p></td></tr><tr><td><p dir="ltr">01_Original_2015_Experimental_Data</p></td><td><p dir="ltr">Original CCM3, Cx43, and GAPDH acquisition files (<i>CCM3KD blot</i><i> </i><i>Cx43 with lanes.scn, CCM3KD blot</i><i> </i><i>Cx43.scn, CCM3KD blot CCM3 with lanes.scn, CCM3KD blot CCM3.scn, CCM3KD blot GAPDH with lanes.scn,</i><i>CCM3KD blot GAPDH.scn)</i>, TIFF exports (<i>CCM3KD blot</i><i> </i><i>Cx43.tiff, CCM3KD blot CCM3.tiff, CCM3KD blot GAPDH.tiff)</i> historical quantitative worksheets (<i>8.20.15 E-cadherin expression mcell pericyte ccm3 siRNA KD update.xlsx</i>), and laboratory presentation (<i>8.21.15 for Anuska meeting.pptx, slide 2</i>)</p></td></tr><tr><td><p dir="ltr">02_Original_2017_Experimental_Data</p></td><td><p dir="ltr">Original Cx37, Cx40, Cx43, and tubulin acquisition files (<i>Cx37 Cx40 blot</i><i> </i><i>Cx37.scn, Cx37 Cx40 blot</i><i> </i><i>Cx40.scn, Cx37 Cx40 blot</i><i> </i><i>Cx43.scn, Cx37 Cx40 blot tubulin.scn</i>) TIFF exports (<i>Cx37 Cx40 blot</i><i> </i><i>Cx37.tiff, Cx37 Cx40 blot</i><i> </i><i>Cx40.tiff, Cx37 Cx40 blot</i><i> </i><i>Cx43.tiff, Cx37 Cx40 blot tubulin.tiff)</i> historical quantitative worksheets (2.19.17 figure one raw data.xlsx, sheet: connexin protein) and laboratory presentation (<i>3.2.17 data consolidation.pptx, slide 2</i>)</p></td></tr><tr><td><p dir="ltr">03_Published_Figure2_and_Author_Reconstruction</p></td><td><p dir="ltr">Published Figure 2 (<i>Fig2.tif</i>), author-prepared Figure 2A (<i>Fig2A.tif</i>) reconstruction, and source-image correspondence documentation</p></td></tr><tr><td><p dir="ltr">04_Quantitative_Analysis</p></td><td><p dir="ltr">Quantitative records organized for examination of Figure 2A, with original measurements and retrospective analyses clearly distinguished, file <i>Connexin protein analysis.pzfx</i></p></td></tr><tr><td><p dir="ltr">05_Scientific_Explanation</p></td><td><p dir="ltr">Full scientific explanation of the original experiments, verified measurements, figure-presentation error, and reconstruction</p></td></tr></table><p dir="ltr"><b>3.

Original Data and Figure Reconstruction</b></p><p dir="ltr">Original acquisition files, historical quantitative worksheets, and laboratory presentations are preserved without retrospective alteration. Multiple representations of the same acquisition are retained for documentation and are not counted as additional biological replicates.</p><p dir="ltr">The published Figure 2 and author-prepared Figure 2A reconstruction are provided separately in Folder 03.

The accompanying source-image correspondence documentation identifies the original images, sample and lane assignments, and preparation of the reconstructed panel.</p><p dir="ltr">The complete scientific explanation is provided in Folder 05.</p><p dir="ltr"><b>4. Data Availability</b></p><p dir="ltr">This repository preserves the original experimental evidence underlying Figure 2A and the author-prepared reconstruction for independent scientific examination.<br><b>Related publication DOI:</b> <u>doi.org/10.1096/fj.201700699r</u></p><p dir="ltr"><b>Prepared by:</b> Anuska V. Andjelkovic, MD, PhD<br>Corresponding author</p><p><br></p>