Dataset of confocal microscopy from plant samples - High-throughput characterization of cortical microtubule arrays response to anisotropic tensile stress

The data set contains Tiff Z-stacks from light-grown hypocotyls and cotyledons of cortical microtubule (CMT) reporter lines either with few or no ablated cells from a time series experiment. This data set was analyzed with a new semi-automated image analysis workflow we have developed to quantify CMTs reorganization in individual cells following an ablation (github.com/VergerLab/MT_Angle2Ablation_Workflow). The dataset in the zip file was analyzed using the scripts on GitHub (github.com/VergerLab/MT_Angle2Ablation_Workflow).

A step by step describes and explains all the scripts of the image analysis procedure. The intermediate data generated by the analysis method can be found on zenodo (doi.org/10.5281/zenodo.7436075). The documentation file Example_2D_Image.tif gives a visual representation from a typical z-stack.