Dataset for supporting a modular autoinduction device for control of gene expression in Bacillus subtilis

We have constructed an autoinduction device for gene expression in Bacillus subtilis based on the LuxR/I quorum sensing system (“A modular autoinduction device for control of gene expression in Bacillus subtilis”, doi: 10.1016/j.ymben.2020.03.012). Here, we present raw and processed data regarding to B. subtilis growth measured as OD600, performed in three different scales: microcultivation on 96-well plates (200 µL), test tube (12 mL), and Erlenmeyer flask (50 mL).

We also present raw and processed data on gene expression measured as GFP fluorescence (485/535 nm), luminescence and riboflavin production. Measurements were performed on a microplate reader Tecan 200 PRO (iControl software) and on spectrophotometer (Thermo Fisher Scientific GENESYS 10S UV-Vis). Processed data are presented as product/OD600, maximum and minimum promoter activity, fold of induction, and the induction OD600.