Effects of different concentrations of inorganic phosphate versus parathyroid hormone on osteoblasts

To investigate the effects of inorganic phosphate (P), parathyroid hormone (PTH), and their combined stimulation on proliferation, apoptosis and bone metabolism regulation of osteoblasts, providing a basis for the prevention and treatment of secondary osteoporosis in MHD patients.The MC3T3-E1 osteoblast cell line was divided into blank control group (CON), 2-4mM P single stimulation group, 10-9~-6M PTH single stimulation group, HP-HPTH combined stimulation group and LP-LPTH, LP-HPTH, HP-LPTH treatment group.

Each group was continuously stimulated for 24 hours. Proliferation activity, apoptosis rates and the expression of BAP, RUNX2, OPG,and RANKL was detected.