Data · dataset · 2020
Dataset for the paper entitled "A genetic map of the response to DNA damage in human cells" by Olivieri M et al. Cell 2020
Listed in Teesside University Research Data Repository
We used pooled CRISPR/Cas9 screens in the human RPE1-hTERT p53-/- cell line against 27 genotoxic agents.
Description
The Dataset herein make the primary data available. The files are:
- Additional genes of interest (discussion and data relating to TMEM2, ESD, USP37, PHF12, BTAF1 and DRAP1)
- Supplementary Raw Data: Data used to make all the graphs in the manuscript
- Folder "CRISPR screen readcount files". Readcounts for the screens undertaken as part of this study.
- Folder "Raw images for immunoblots": uncropped images for all the blots in the manuscript
- Folder: "Additional QC analyses of CRISPR screens": Additional analyses that measure the quality of the screens using Presion-Recall curves of essential genes and an estimate of genes that scored as hits due to batch effects.
- Folder "Data_Analysis_Code" R markdown file along with data files
- File: "Additional Genes of Interests" contains a short discussion of genes we found noteworthy after analysis of our screen data. Version 2 note: this version differs from Version 1 by a single file (FDRPos_31screens.csv) which has a tab error fixed.
Links
Where it is published
- DOI doi.org/10.17632/gfcn2wmrpf.2 ↗
DOI / persistent id · from researchdata tees ac uk
Catalogue records · 1
- OAI-PMH record data.mendeley.com/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Adata… ↗
metadata API · from researchdata tees ac uk
Topics
- From keywords
- Data quality · Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Image 65%
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Teesside University Research Data Repository | oai:data.mendeley.com/gfcn2wmrpf.2 | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].anzsrc:field:460504 | mapping · researchdata tees ac uk | vocabulary-mapper@1.0.0 | keywords['Data Quality'] |
| concepts[field].local:field:earth-environmental | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:engineering | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:humanities | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:social-science | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[modality].local:modality:image | enrichment · researchdata tees ac uk | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| title | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/title |