Data · dataset · 2025
Raw dataset of light-sheet-microscopy images for a mouse brain sample labeled for Dorsal Raphe Nucleus serotonergic neurons
Listed in ScienceDB
Comprehensive mapping of neuronal connections across entire nervous systems remains a fundamental challenge in neuroscience.
Description
Here, we introduce LINCS (Labeling Individual Neurons with Chemical dyes and controllable Sparseness), a technology that achieves rapid, ultrabright, and photostable labeling of specific cell types throughout the entire mouse brain and body. LINCS utilizes an engineered, solubility-enhanced biotin ligase for in vivo biotinylation, followed by rapid whole-mount staining with a high-affinity monovalent streptavidin.
When integrated with tissue clearing and light-sheet microscopy, this system creates an efficient pipeline for profiling long-range neuronal projections across both the central and peripheral nervous systems. This is a raw dataset (tiff files) of a LINCS whole-brain sample (DRN serotonergic neurons) and its downsampled version (factor: 0.125). More detail is described in the README pdf file.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.25504 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Biochemistry and cell biology 74% · Image 65% · Microscopy 75%
Provenance · 1 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.25504 | 12 d ago | JSON v1 |
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|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].anzsrc:group:3101 | enrichment · scidb cn | taxonomy-embedding@1.0.0 | title+keywords+description (74%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
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| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:image | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:microscopy | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |