Source Data for A pathogen-derived small RNA modulates host immunity for chronic parasitism

The counting files include embedded Excel formulas demonstrating the conversion from raw hemocytometer counts to final concentrations.Image Data: A sub-folder containing original, unaltered microscopy images (captured at 10x magnification) for Fig. 5D.Fig.6_data:Target Prediction: Numerical results of miRanda target site predictions for the attacin 3'UTR across various species (Fig. 6A).Phylogenetic Data: Raw Newick (.nwk) files for the phylogenetic trees constructed using CDS (Conserved) and 3'UTR (Divergent) sequences (Fig. 6B).Nucleotide Diversity: Raw numerical data and calculated Pi (π) values supporting the nucleotide diversity analysis shown in Fig. 6C.Species coverage: species number in every midpoint (Fig.6D).Sequence Data: A compiled text file containing the 3'UTR sequences of attacin from multiple species used for sequence alignment and target site conservation analysis (Fig. 6E).Fig.S1_data Correlation of Os-milRNA2 and attacin-1Fig.S2_dataLuciferase Assay: Raw Firefly and Renilla bioluminescence readings and normalized ratios for Figure S2B.qPCR Data: Zinc transpoter ΔCt values and 2−ΔΔCt calculations for Fig. S3C.Table S1.

List of primer sequences used for quantitative real-time PCR (qPCR) analysis.Table S2. Genome mapping analysis of identified Os-milRNAs.Table S3. Functional annotations of predicted target genes for Os-milRNA1.Table S4.

Functional annotations of predicted target genes for Os-milRNA2.Table S5. miRanda-based binding affinity prediction of Os-milRNA2 binding sites across various insect species.Table S6. In silinco specificity analysis of Antagomir and NC sequences.Data Standards:Statistical Calculations: All P-values were derived from Student’s t-tests or One-way ANOVA with Tukey’s post-hoc tests as specified in the Excel files.Image Integrity: All provided images are original, high-resolution, and have not undergone any manipulative adjustments.Accession: All analyses are based on raw sequencing data deposited in the Genome Sequence Archive (GSA) under accession number CRA038699.