Transcriptomic and proteomic profiling of spinal cord tissues from SOD1-G93A mice treated with peptide JP1

This dataset comprises transcriptomic and proteomic profiles of spinal cord tissues from SOD1-G93A transgenic mice, a well-established model of amyotrophic lateral sclerosis (ALS), following treatment with JP1, a synthetic peptide derived from the JWA protein that activates the Keap1-Nrf2-ARE signaling pathway.Spinal cord samples were collected from three experimental groups at the symptomatic stage (120 days): untreated wild-type (WT) mice, untreated SOD1-G93A mice (ALS model), and SOD1-G93A mice treated with JP1 (150 mg/kg, intraperitoneal injection).

Transcriptomic analysis was performed using RNA sequencing (RNA-seq), and proteomic analysis was conducted using high-throughput liquid chromatography-tandem mass spectrometry (LC-MS/MS).The dataset is intended to elucidate the molecular mechanisms underlying JP1-mediated neuroprotection in ALS, with a focus on the coordinated regulation of oxidative stress and autophagy via the Keap1-Nrf2-ARE pathway. Raw and processed data are provided for both transcriptomic and proteomic analyses, enabling comprehensive exploration of differentially expressed genes and proteins, pathway enrichment, and integrative multi-omics analyses.These data support the findings reported in the associated study, which demonstrates that JP1 improves motor function, extends survival, and mitigates neurodegeneration in ALS mice by restoring autophagic flux and alleviating oxidative stress through Nrf2 pathway activation.