Excel · study · 2026
miRNA Sequencing of porcine-derived extracellular vesicles
Listed in NCBI GEO
Extracellular vesicles (EVs) have gained significant attention as therapeutics, building from natural mechanisms of paracrine signaling.
Description
Despite this interest, there is substantial heterogeneity in methods to isolate and characterize EVs and new methods are needed to scale-up EV production to obtain the quantities necessary for therapeutic use. In this study, we isolated EVs from four porcine donors of bone-marrow derived mesenchymal stromal cells (MSCs) via three different cell culture methods: standard tissue culture plates, a 3D printed perfusion bioreactor, and microcarriers in spinner flasks.
We then explored EV characteristics and yield, RNA and protein content as a function of biological donor for bone marrow-derived MSCs and cell culture conditions in order to understand the effect of biological donor and cell culture parameters.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE303nnn/GSE303752 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE303752 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1297619 ncbi.nlm.nih.gov/bioproject/PRJNA1297619 ↗
project · from NCBI GEO
- PubMed 41320582 pubmed.ncbi.nlm.nih.gov/41320582 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Non-coding RNA profiling by high throughput sequencing · Sus scrofa
- From keywords
- Life Sciences
- Inferred from text
- Sequencing 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE303752 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:non-coding-rna-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9823 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |