Climate variability in the northern Mediterranean across the onset of the Messinian salinity crisis: insights from inorganic and organic geochemical proxies

Lipid biomarker analyses targeted long-chain n-alkanes, derived from leaf waxes of land plants, which were identified and quantified, allowing to trace terrestrial organic matter input in the study area. We calculated the Carbon Preference Index (Bryan and Evans, 1961) based on the concentration of the n-C24 to n-C34 alkanes to trace the level of degradation/contamination of the terrestrial organic matter. Finally, carbon (δ13C) and hydrogen (δ2H) stable isotopes of the n-C31 and n-C33 alkanes were determined. δ13C values allowed to reconstruct variations in vegetation (C3 vs C4 plants), while δ2H values helped in reconstructing the hydrological cycle at the onset of the Messinian salinity crisis in the northern Mediterranean.

Bray, E.E., Evans, E.D., 1961. Geochimica and Cosmochimica Acta 22, 2–15. doi.org/10.1016/0016-7037(61)90069-2