<p>Comparison of Gene Induction in Response to IFNγ in Individuals with Asymptomatic Versus Ulcerative HSV-2 Disease.</p>

Each line represents the average of a single donor. Gene expression normalized to β-actin (dashed line).

Data is represented as dCt, which is calculated by subtracting the Ct value of β-actin from the Ct of induced genes. All Ct values were acquired directly from qPCR with a maximum Ct threshold of 35. <b>(B)</b> Fold change in <i>CXCL10, CCL5, IL15, IL12A</i>, and <i>IFI16</i> expression calculated as relative to mock expression, normalized to β-actin, calculated using the ΔΔCt method 4 hours post treatment with IFN-γ. Darker bars represent asymptomatic cohort and lighter shade bars represent ulcerative cohort. <b>(C)</b> Fold change in <i>CXCL10, CCL5, IL15, IL12A</i>, and <i>IFI16</i> expression in asymptomatic (dark bars) and ulcerative cohorts at 4 and 24 hours post treatment with 100 U/mL IFNγ.