Text · study · 2026
PEDV Discontinuous Transcription Generates A Novel Subgenomic RNA to Enhance Immune Evasion and Viral Fitness
Listed in NCBI GEO
During viral evolution, RNA viruses accumulate advantageous mutations that enhance replicative, adaptability, and evasion of host immune responses.
Description
While most studies have focused on amino acid substitutions that alter protein function, functional RNA-level mutations remain largely underexplored. In this study, we employed RNA-seq and Ribo-seq for the first time to analyze viral gene expression during replication of the porcine epidemic diarrhea virus (PEDV).
We identified a novel subgenomic RNA (sgRNA) that encodes a truncated nucleocapsid isoform, designated N1. Evolutionary analysis revealed that a double-point mutation within the GII-b subclade facilitated the emergence of a functional transcriptional regulatory sequence (TRS-B) and a translation initiation codon. N1 promotes viral replication but is unable to substitute for the full-length N protein in the assembly of infectious virions.
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Mechanistically, N1 binds double-stranded RNA (dsRNA) and suppresses dsRNA-induced phosphorylation of protein kinase R (PKR) and eukaryotic translation initiation factor 2α (eIF2α), thereby inhibiting the formation of host stress granules (SG). Mutations at residues R133 and R134 markedly impair the dsRNA-binding capacity of N1 and its ability to inhibit SG formation. Further transcriptomic analysis of infected cells revealed that the absence of N1 enhances activation of host innate immune response following viral infection, thereby suppressing viral replication.
Our findings demonstrate that PEDV can promote the generation of novel innate immune antagonists through the evolution of TRS-B, thereby enhancing its immune evasion ability and adaptation to the host.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE330nnn/GSE330201 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE330201 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1463113 ncbi.nlm.nih.gov/bioproject/PRJNA1463113 ↗
project · from NCBI GEO
Topics
- Stated by source
- Other
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65%
Related
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE330201 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |