Data · dataset · 2026
CUT&Tag-seq data of ZmHsfA5-overexpressing maize plants for article "Heat shock transcription factor ZmHsfA5 coordinates with ZmHsp9 to enhance thermotolerance in maize"
Listed in ScienceDB
Nuclear extracts were isolated from leaves of ZmHsfA5-myc OE lines at the three-leaf stage. samples were collected at 45 °C for 0 h and 45 °C for 3 h, respectively. Each biological replicate comprised 1.0 g leaf tissue (n = 2 replicates per group).
Description
Protein-DNA complexes were captured using the a CUT&Tag® assay kit (TD901-01, Vazyme). The experimental group employed an anti-myc antibody (ab32072, Abcam, UK), while whereas the control group used an anti-IgG antibody.
Sequencing libraries were constructed following manufacturer's instructions and sequenced with 150-bp paired-end reads on an Illumina NovaSeq 6000 platform (GENEWIZ, Suzhou, Jiangsu, China).
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.33230 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.33230 | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license_text | source · scidb cn | connector:scidb_cn@1.0.0 | |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |