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Table · dataset · 2026

Supplementary file 1_Morphological characterization and multilocus phylogenetic placement of Physalia utriculus (Siphonophore: Physaliidae) from the Gulf of Thailand.docx

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<p>Physalia is a group of pleustonic siphonophores renowned for wind-current driven dispersal, and is a recurring public-health and tourism concern on Thai beaches.

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Addressing the problem has been hindered by the unresolved taxonomy and distribution of Physalia in Thai waters for decades. This study provides the specimen-based multilocus characterization of Physalia utriculus from the Gulf of Thailand, complementing earlier morphology-based Thai records, by integrating morphological characterization with multi-gene (16S, 18S, and COI) analysis and a COI haplotype network.

Specimens collected from Chalathat and Samila beaches of the Songkhla province, exhibited the following diagnostic features: an asymmetrical blue-green triangular pneumatophore with a rugose crest (15–38 wrinkles), one principal tentacle, and flask-shaped gastrozooids. Phylogenetic reconstruction placed the Thai specimens within the global clade of P. utriculus, supporting their assignment to this species on the basis of phylogenetic affinity.

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The median-joining COI haplotype network of 85 Indo–Pacific and Atlantic individuals resolved 56 haplotypes. Thai specimens shared the two most common, geographically widespread haplotypes with other Indo–Pacific populations but also carried a high proportion (11 of 13) of locally restricted haplotypes. Analysis of molecular variance revealed no significant COI differentiation was detected among the four well-sampled Indo-Pacific regions, although this inference is constrained by uneven geographic sampling and the inclusion of only one Atlantic sequence.

Monthly mean wind and surface-current fields indicate that the February 2026 stranding occurred under northeast-monsoon conditions, but these climatological summaries do not identify a source region or reconstruct individual transport trajectories. Together, the morphological and multilocus data provide a specimen-based baseline for P. utriculus in the Gulf of Thailand.</p>

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Evolutionary biology 72%
Provenance · 1 source records, 17 field assertions
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