Data · dataset · 2026
<p>Raw image of western blot used in S1C Fig.</p>
Listed in UCL Research Data Repository
<p>Lysates of cells transfected with bat Sarbecovirus Spikes were boiled in nonreducing loading buffer for 5 min.
Description
SDS-PAGE and membrane-transfer was conducted as per standard methods. The membrane was blocked and incubated with primary mouse anti-FLAG tag antibody.
For tagged Sarbecovirus Spikes, Spike expression was detected using secondary anti-mouse antibody, at expected band size of ~180 kDa. The blot was captured using LiCor Odyssey Fc using 700 nm (ladder) and 800 nm (sample) channel. The membrane was subsequently incubated with anti-GAPDH antibody and detected using a similar method at expected size of 36 kDa.</p> <p>(TIF)</p>
Links
Where it is published
- DOI doi.org/10.1371/journal.pbio.3003882.s011 ↗
DOI / persistent id · from rdr ucl ac uk
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from rdr ucl ac uk
Topics
- From keywords
- Earth & Environmental Science · Ecology · Evolutionary biology · Immunology · Infectious diseases · Life Sciences · Medicine & Health · Microbiology · Social Science · Virology
- Inferred from text
- Image 75%
Provenance · 1 source records, 16 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| UCL Research Data Repository | oai:figshare.com:article/34048930 | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].anzsrc:field:310706 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Virology'] |
| concepts[field].anzsrc:field:320211 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Infectious Diseases'] |
| concepts[field].anzsrc:group:3103 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Ecology'] |
| concepts[field].anzsrc:group:3104 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Evolutionary Biology'] |
| concepts[field].anzsrc:group:3107 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].anzsrc:group:3204 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Immunology'] |
| concepts[field].local:field:earth-environmental | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:social-science | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[modality].local:modality:image | enrichment · rdr ucl ac uk | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| title | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/title |