Gene annotation of Blastobotrys mokoenaii, Blastobotrys illinoisensis, and Blastobotrys malaysiensis

The genome assemblies for B. mokoenaii (NRRL Y-27120) and B. malaysiensis (NRRL Y-6417) were publicly available on the National Center for Biotechnology Information (NCBI) under accessions GCA_003705765.3 and GCA_030558815.1, respectively. The genome assembly for B. illinoisensis (NRRL YB-1343) was generated by SciLifeLab's National Genomics Infrastructure (NGI) using PacBio long-read data and deposited in the European Nucleotide Archive (ENA) under accession GCA_965113335.1.

File description- bmokoenaii_annotation.gff This file contains the gene models predicted for B. mokoenaii (GCA_003705765.3). - billinoisensis_annotation.gff This file contains the gene models predicted for B. illinoisensis (GCA_003705765.3). - bmalaysiensis_annotation.gff This file contains the gene models predicted for B. malaysiensis (GCA_030558815.1). Gene annotation methodsRepeat MaskingPrior to annotation, a repeat library was built for each species using RepeatModeler2 v2.0.2 and the genomes were soft-masked using RepeatMasker v4.1.5. $ RepeatModeler -database ${DB} -engine ncbi -pa 16 $ RepeatMasker -dir . -gff -u -no_is -xsmall -e ncbi -lib ${LIBRARY} -pa 16 genome.fasta Structural Annotation Structural annotation was performed on the soft-masked genomes using Braker3 v3.0.3 incorporating external evidence in the form of all fungal proteins from OrthoDB v11 (available at bioinf.uni-greifswald.de/bioinf/partitioned_odb11). $ braker.pl --genome="$genome" \ --prot_seq=${protein} --workingdir=${PWD} \ --gff3 --threads=16 --verbosity=3 \ --nocleanup --species=${i} Functional Annotation The predicted genes were functionally annotated using the National Bioiformatics Infrastructure Sweden (NBIS) functional_annotation nextflow pipeline v2.0.0 (github.com/NBISweden/pipelines-nextflow).