Data · dataset · 2026
Reduced Representation Bisulfite Sequencing library (RRBS) preparation of SAM-treated A549
Listed in ScienceDB
Genomic DNA (gDNA) was extracted from A549 cells following SAM treatment (8 hrs) using the Wizard Genomic DNA Purification kit (Promega).
Description
Purified gDNA (1 μg) was fragmented to an average size of 400 bp using a Covaris LE220R sonicator. Fragmented DNA was ligated to methylated adapters, followed by bisulfite treatment using the DNA Bisulfite Conversion Kit (Beyotime) according to the manufacturer’s protocol.
The resulting single-stranded libraries were amplified using KAPA HiFi HotStart Uracil+ ReadyMix (Roche). Library concentration and size distribution were validated using a Qubit fluorometer (Thermo Fisher Scientific) and an Agilent 2100 Bioanalyzer, respectively. RRBS was performed on an Illumina NovaSeq 4000 platform in 150 bp paired-end mode
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.33414 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Biochemistry and cell biology 68% · Sequencing 75%
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.33414 | 7 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].anzsrc:group:3101 | enrichment · scidb cn | taxonomy-embedding@1.0.0 | title+keywords+description (68%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |