Efficient Production of 3'-Sialyllactose by Single Whole-Cell in One-Pot Biosynthesis

By using the optimal conditions (35 C, pH 7.0, 20 mMpolyphosphate, 10 mM cytidine monophosphate (CMP), 20 mM MgCl2), 25 mL and 4 L conversionsystems were carried out to produce 3'-SL. Similar results were obtained between different volumeconversion reactions, which led the maximum production of 3'-SL to reach 53 mM from 54.2 mMof sialic acid (SA) in the 25 mL system and 52.8 mM of 3'-SL from 53.8 mM of SA in the 4 L system.These encouraging results demonstrate that the developed single whole-cell multi-enzyme systemexhibits great potential and economic competitiveness for the manufacture of 3'-SL.