Replication Data for: "Host transcriptional signatures associated with disease tolerance and environmental persistence in a mosquito–microsporidian system"

Here, experimental evolution and RNA sequencing were combined in the mosquito–microsporidian system Anopheles gambiae–Vavraia culicis to test whether parasite lineages selected for early or late transmission differ in the host responses they elicit. Early lineages were selected from hosts that died early in infection, whereas late lineages were selected from hosts that died later, generating parasite populations that differ in growth, virulence and environmental persistence.

Mosquitoes were infected with evolved parasite lineages and sampled at a common sporulating stage. A reference infection response was defined relative to uninfected controls. Early- and late-selected parasites showed consistent, yet partly distinct, changes in host gene expression.