A bioactive peptide from the skin secretion of the Chinese Torrent Frog, Amolops wuyiensis

A variety of bioactive components with unique structures and functions which isolated from the skin secretion, especially the peptides, have enormous potential for medical purposes. <br>Here, a Kunitz-type peptide was identified from the Chinese Torrent Frog, Amolops wuyiensis by molecular cloning.

Then the mature peptide was successfully synthesised by Tribute® 2-channel peptide synthesiser. Reversed-phase high performance liquid chromatography (RP-HPLC) was used for purification and matrix-assisted laser desorption ionisation time-of-flight (MALDI-TOF) MS was used to confirm its molecular mass. This peptide was found to show relatively strong trypsin inhibition with a Ki value of 2.331 µM with weak hemolysis at a concentration up to 512μM. The peptide was ineffective against the gram-positive bacterium S.aureus, gram-negative bacterium E.coli and pathogenic yeast C.albicans.