Development and Application of Long-Fragment Insertion/Deletion Markers in Ficus carica L.

Title: Development and Application of Long-Fragment Insertion/Deletion Markers in Ficus carica L.Corresponding author: Zhenlei WangContact: wangzhenlei@xxx.comDataset description:This dataset contains supplementary tables and images supporting the development and validation of 56 long‑fragment InDel markers in fig (Ficus carica L.). Data include resequencing quality, InDel distribution, primer information, DNA fingerprints, and gel/population structure images.File list & descriptionData file 1: Sequencing statistics.xlsxType: Spreadsheet (.xlsx)Content: Whole‑genome resequencing quality metrics for four fig accessions (Hongyan, Jin Aofen, Bojihong, Zaohuang), including Q30, GC content, genome coverage, and sequencing depth.Data file 2: InDel distribution by chromosome.xlsxType: Spreadsheet (.xlsx)Content: Number of InDel variants detected on each of the 13 fig chromosomes.Data file 3: Genomic region distribution.xlsxType: Spreadsheet (.xlsx)Content: Distribution of InDels across genomic functional regions (upstream, downstream, intron, exon, UTR, intergenic).Data file 4: InDel length distribution (≥20 bp).xlsxType: Spreadsheet (.xlsx)Content: Frequency distribution of long‑fragment InDels (≥20 bp) by size class (20–280 bp).Data file 5: Primer sequences and polymorphism indices.xlsxType: Spreadsheet (.xlsx)Content: Primer sequences, chromosomal positions, PIC values, and Shannon diversity indices for the 56 validated InDel markers.Data file 6: DNA fingerprint codes.xlsxType: Spreadsheet (.xlsx)Content: 56‑digit binary fingerprint codes for 28 fig accessions using the validated InDel marker set.Data file 7: Population structure results.pngType: Image (.png)Content: Admixture proportion bar plot (K=6) for 28 fig accessions, generated by STRUCTURE 2.3.4.Data file 12: InDel length distribution figure.pngType: Image (.png)Content: Mirrored bar chart showing size distribution of InDels in CDS and whole‑genome contexts across four accessions (R1–R4).Data file 13: Agarose gel electrophoresis.pngType: Image (.png)Content: Electrophoresis images for 26 representative InDel markers (A–Z) across 28 fig accessions.Methodology summaryResequencing platform: Illumina NovaSeq 6000 (2 × 150 bp)Reference genome: Ficus carica UNIPI_FiCari_1.0Variant calling: GATK v3.8Marker screening: 2% agarose gel electrophoresisGenetic diversity: Popgene 32Population structure: STRUCTURE 2.3.4 (K=6, ΔK method)