Table · dataset · 2026
Data Sheet 1_Extraction-free LAMP-LFD assay for early diagnosis of congenital Chagas disease directly from neonatal dried blood spots.pdf
Listed in ZivaHub and Deakin Research Online and DMU Figshare and HKU DataHub and Swinburne Figshare and DaYta Ya Rona and SUNScholarData and figshare and Loughborough Research Repository and GRANTS Data and UP Research Data Repository — shown once because both records carry DOI 10.3389/fcimb.2026.1934037.s001
Background<p>Vertical transmission is a major driver of new Chagas disease cases.
Description
Early diagnosis in newborns is essential for effective treatment; however, conventional screening limitations and high patient attrition lead to severe under-registration. Simplified molecular tools can substantially improve early detection and treatment access.</p>Methodology/principal findings<p>We developed a simplified loop-mediated isothermal amplification (LAMP)-based assay targeting the repetitive satellite DNA of Trypanosoma cruzi for qualitative detection in newborn dried blood spots (DBS).
Tailored for resource-limited settings, the protocol requires only a dry bath incubator, utilizing lateral flow dipsticks (LFD) for visual readout. The DBS sample is loaded directly into the reaction without nucleic acid extraction. Using artificially spiked DBS, the limit of detection was established at 1 fg of DNA and 1 parasite per reaction.
Read the rest (2 more)
Inclusivity was confirmed across all six T. cruzi discrete typing units (DTUs TcI–TcVI), with no cross-reactivity against heterologous or human DNA. A clinical proof-of-concept evaluation on a blinded panel of 24 newborn DBS samples (4 positive, 20 negative) achieved 100% concordance with definitive clinical status. Notably, the assay successfully identified all 4 congenital cases, including 3 that had tested negative by conventional parasitological microscopy at birth.</p>Conclusions/significance<p>This simplified, extraction-free molecular test requires minimal equipment and is fully compatible with routine newborn screening infrastructure.
It provides a robust, translatable tool for early congenital Chagas diagnosis in decentralized healthcare settings, with high potential to improve detection rates and secure timely access to etiological treatment.</p>
Links
Where it is published
- DOI doi.org/10.3389/fcimb.2026.1934037.s001 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Astronomy & Astrophysics · Chemistry · Chemistry · Chemistry · Clinical microbiology · Clinical microbiology · Clinical microbiology · Clinical microbiology · Clinical microbiology · Clinical microbiology · Clinical microbiology · Clinical microbiology · Clinical microbiology · Clinical microbiology · Clinical microbiology · Computer Science & AI · Computer Science & AI · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Economics & Finance · Economics & Finance · Engineering · Engineering · Engineering · Engineering · Humanities · Humanities · Humanities · Humanities · Humanities · Life Sciences · Life Sciences · Life Sciences · Life Sciences · Life Sciences · Life Sciences · Life Sciences · Life Sciences · Life Sciences · Life Sciences · Life Sciences · Materials Science · Mathematics & Statistics · Medicine & Health · Medicine & Health · Medicine & Health · Medicine & Health · Medicine & Health · Medicine & Health · Medicine & Health · Medicine & Health · Medicine & Health · Medicine & Health · Medicine & Health · Ocean & Atmospheric Science · Psychology & Behavioral Science · Psychology & Behavioral Science · Social Science · Social Science · Social Science · Social Science · Social Science
- Inferred from text
- Disease 75% · Microscopy 75%
Provenance · 11 source records, 78 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/34038852 | 12 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/34038852 | 12 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/34038852 | 12 d ago | JSON v1 |
| HKU DataHub | oai:figshare.com:article/34038852 | 12 d ago | JSON v1 |
| Swinburne Figshare | oai:figshare.com:article/34038852 | 12 d ago | JSON v1 |
| DaYta Ya Rona | oai:figshare.com:article/34038852 | 12 d ago | JSON v1 |
| SUNScholarData | oai:figshare.com:article/34038852 | 12 d ago | JSON v1 |
| figshare | oai:figshare.com:article/34038852 | 11 d ago | JSON v1 |
| Loughborough Research Repository | oai:figshare.com:article/34038852 | 11 d ago | JSON v1 |
| GRANTS Data | oai:figshare.com:article/34038852 | 11 d ago | JSON v1 |
| UP Research Data Repository | oai:figshare.com:article/34038852 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].anzsrc:field:320203 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Clinical Microbiology'] |
| concepts[field].anzsrc:field:320203 | mapping · datahub hku hk | vocabulary-mapper@1.0.0 | keywords['Clinical Microbiology'] |
| concepts[field].anzsrc:field:320203 | mapping · figshare swinburne edu au | vocabulary-mapper@1.0.0 | keywords['Clinical Microbiology'] |
| concepts[field].anzsrc:field:320203 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Clinical Microbiology'] |
| concepts[field].anzsrc:field:320203 | mapping · scholardata sun ac za | vocabulary-mapper@1.0.0 | keywords['Clinical Microbiology'] |
| concepts[field].anzsrc:field:320203 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Clinical Microbiology'] |
| concepts[field].anzsrc:field:320203 | mapping · dayta nwu ac za | vocabulary-mapper@1.0.0 | keywords['Clinical Microbiology'] |
| concepts[field].anzsrc:field:320203 | mapping · researchdata up ac za | vocabulary-mapper@1.0.0 | keywords['Clinical Microbiology'] |
| concepts[field].anzsrc:field:320203 | mapping · grantsdata jst go jp | vocabulary-mapper@1.0.0 | keywords['Clinical Microbiology'] |
| concepts[field].anzsrc:field:320203 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Clinical Microbiology'] |
| concepts[field].anzsrc:field:320203 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Clinical Microbiology'] |
| concepts[field].local:field:astronomy | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · scholardata sun ac za | connector:scholardata_sun_ac_za@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:computer-science-ai | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:computer-science-ai | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · scholardata sun ac za | connector:scholardata_sun_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · researchdata up ac za | connector:researchdata_up_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · grantsdata jst go jp | connector:grantsdata_jst_go_jp@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · datahub hku hk | connector:datahub_hku_hk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare swinburne edu au | connector:figshare_swinburne_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dayta nwu ac za | connector:dayta_nwu_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:economics-finance | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:economics-finance | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:engineering | mapping · researchdata up ac za | connector:researchdata_up_ac_za@1.0.0 | |
| concepts[field].local:field:engineering | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:engineering | mapping · dayta nwu ac za | connector:dayta_nwu_ac_za@1.0.0 | |
| concepts[field].local:field:engineering | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:humanities | mapping · researchdata up ac za | connector:researchdata_up_ac_za@1.0.0 | |
| concepts[field].local:field:humanities | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:humanities | mapping · dayta nwu ac za | connector:dayta_nwu_ac_za@1.0.0 | |
| concepts[field].local:field:humanities | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scholardata sun ac za | connector:scholardata_sun_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · dayta nwu ac za | connector:dayta_nwu_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scholardata sun ac za | connector:scholardata_sun_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · researchdata up ac za | connector:researchdata_up_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare swinburne edu au | connector:figshare_swinburne_edu_au@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · datahub hku hk | connector:datahub_hku_hk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · grantsdata jst go jp | connector:grantsdata_jst_go_jp@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:materials-science | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:mathematics-statistics | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · scholardata sun ac za | connector:scholardata_sun_ac_za@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · dayta nwu ac za | connector:dayta_nwu_ac_za@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · figshare swinburne edu au | connector:figshare_swinburne_edu_au@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · datahub hku hk | connector:datahub_hku_hk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · grantsdata jst go jp | connector:grantsdata_jst_go_jp@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · researchdata up ac za | connector:researchdata_up_ac_za@1.0.0 | |
| concepts[field].local:field:ocean-atmospheric | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:psychology-behavioral | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:psychology-behavioral | mapping · scholardata sun ac za | connector:scholardata_sun_ac_za@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scholardata sun ac za | connector:scholardata_sun_ac_za@1.0.0 | |
| concepts[field].local:field:social-science | mapping · dayta nwu ac za | connector:dayta_nwu_ac_za@1.0.0 | |
| concepts[field].local:field:social-science | mapping · researchdata up ac za | connector:researchdata_up_ac_za@1.0.0 | |
| concepts[field].local:field:social-science | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:social-science | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[modality].local:modality:microscopy | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/rights |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |