P29-20 Establishing realistic dry aerosol exposure conditions at the Air-Liquid Interface with an in vitro alveolar model [* Cross-Reference *]

Deposition of the aerosolized materials onto the cellular surface was quantified by two independent methods: gravimetric analysis and surface-sensitive quartz crystal microbalance (sQCM). Scanning electron microscopy (SEM) was used to qualitatively confirm particle localization. Cell viability was assessed using the Alamar Blue assay, while intracellular reactive oxygen species (ROS) production was measured via flow cytometry.

Pro-inflammatory cytokine release was analyzed in both apical and basolateral compartments using Luminex-based multiplexing. In addition, particle–cell interactions and internalization were visualized through light microscopy on Richardson-stained cell cross-sections. Even though the PowderX device allows only limited throughput, with just four wells exposed at a time, making it less suitable for large-scale screening, it enables accurate and reproducible dose-response curves for dry powder exposure, demonstrating its suitability for studies requiring realistic exposure scenarios without the complications of dispersion protocols. ***This entry has been automatically imported via BNL CSV by LIST harvest scripts.

Please refer to doi.org/10.1016/j.toxlet.2025.07.861 for the original and latest version of the publication***