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Data · dataset · 2024

Tumor-Associated Schwann Cell Remodeling under Metabolic Stress via Lactate Sensing Orchestrates

Listed in Teesside University Research Data Repository

Biotin pull-down assay The Magic Dynabeads MyOne Streptavidin T1 were pre-incubated with free biotin or biotinylated lactate at room temperature for 30 minutes to saturate the bead surface with biotin.

Description

Subsequently, the pre-treated beads were mixed with the cell lysate of interest and incubated overnight at 4°C with rotation. The beads were then washed four times with PBS buffer to remove non-specifically bound molecules.

After washing, the proteins were separated from the beads, and the presence and expression levels of the target protein were detected using techniques such as Western blotting or proteomics.

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Where it is published

Catalogue records · 1

Topics

From keywords
Cancer · Earth & Environmental Science · Mass spectrometry

Related

Provenance · 1 source records, 8 field assertions
SourceKeyLast seenRaw
Teesside University Research Data Repositoryoai:data.mendeley.com/nttsc8p85p.116 h agoJSON v1
FieldAssertionExtractorEvidence
access_levelsource · researchdata tees ac ukconnector:researchdata_tees_ac_uk@1.0.0
concepts[disease].local:disease:cancermapping · researchdata tees ac ukvocabulary-mapper@1.0.0keywords['Tumor']
concepts[field].local:field:earth-environmentalmapping · researchdata tees ac ukconnector:researchdata_tees_ac_uk@1.0.0
concepts[modality].local:modality:mass-spectrometrymapping · researchdata tees ac ukvocabulary-mapper@1.0.0keywords['Proteomics']
descriptionsource · researchdata tees ac ukconnector:researchdata_tees_ac_uk@1.0.0/metadata/dc/description
licensesource · researchdata tees ac ukconnector:researchdata_tees_ac_uk@1.0.0/metadata/dc/rights
publication_datesource · researchdata tees ac ukconnector:researchdata_tees_ac_uk@1.0.0
titlesource · researchdata tees ac ukconnector:researchdata_tees_ac_uk@1.0.0/metadata/dc/title