Structure · dataset · 2026
<p>Z-disc Adhirons are specific for their targets.</p>
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<p>A and B show the results from Alphafold3 modelling for the Adhirons for Z1Z2 (A) and ZASP (B) with their target proteins.
Description
The structures are shown in Alphaphofold3 colours, to show the prediction accuracy and as ribbon structures to show potential sites of interaction. C. The specificity of the Z-disc Adhirons for their target proteins was confirmed by immunoprecipitations using the Adhiron His-Tag, His-Tag Dynabeads and GST-tagged target proteins, followed by immunoblotting for GST and His Tags.
All Adhirons bound their targets with minimal cross-reactivity and no Adhirons bound to the End Z2 to Zr5 Titin protein. Ad – Adhiron, Cont. – control (an Adhiron with AAAA and AAE in the variable loops). Representative blots shown (S1 Raw Gel).
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N = 3. D. Example SPR data. Adhirons were immobilized on streptavidin-coated Series S sensor chips via C‑terminal biotin and differing concentrations of GST-ZASP PDZ, GST-Z1/Z2, GST‑α‑actinin‑2 CH domains and GST-end Z2 to Zr5 flowed over.
Representative curves are shown. No binding was observed for GST- α‑actinin‑2 CH domains in this assay.</p> <p>(TIF)</p>
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Where it is published
- DOI doi.org/10.1371/journal.pcbi.1014309.s001 ↗
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Topics
- From keywords
- Biochemistry and cell biology · Chemistry · Earth & Environmental Science · Immunology · Inorganic chemistry · Life Sciences · Plant biology
Provenance · 1 source records, 12 field assertions
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|---|---|---|---|
| ZivaHub | oai:figshare.com:article/34017926 | 12 d ago | JSON v1 |
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| concepts[field].anzsrc:group:3108 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Plant Biology'] |
| concepts[field].anzsrc:group:3204 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Immunology'] |
| concepts[field].anzsrc:group:3402 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Inorganic Chemistry'] |
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| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |