Endometrial transcriptomic responses associated with a 17α-hydroxyprogesterone caproate-containing formulation and progesterone in Holstein cows: an exploratory RNA-seq analysis

In this exploratory RNA-seq study, six Holstein cattle were assigned to a 17-OHPC-containing formulation group (100 mg 17-OHPC plus 10 mg progesterone [P4]; n = 3) or a P4-alone group (100 mg P4; n = 3).

Endometrial samples were collected during an untreated synchronized cycle on days 4 and 7, and during a second synchronized cycle on day 7 after treatment on day 4. RNA-seq was used to characterize treatment-associated endometrial transcriptomic profiles and gene-set enrichment patterns between the 17-OHPC/P4 formulation and P4-alone arms.