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Data · collection · 2016

Production and properties of inulinase from Penicillium sp . NFCC 2768 grown on inulin-rich vegetal infusions

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Inulinase production by Penicillium sp.

Description

NFCC 2768 isolated from the rhizosphere soil of dahlia was studied on media containing inulin-rich plant extracts. The maximum inulinase activity (64.54 nkat/ml) was observed with the tuber extract of dahlia ( Dahlia pinnata ).

The fungus produced substantial inulinase activity on asparagus root powder (45.23 nkat/ml) and garlic extracts (41.32 nkat/ml). The apparent molecular weight of the purified inulinase was 68 kDa. The optimum pH and temperature for enzyme activity were 5.0 and 50°C, respectively.

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Mn 2+ and Ca 2+ were found to enhance the inulinase activity, while Hg 2+ was found to be a strong inhibitor. Inulinase liberated fructose, glucose, sucrose, kestose (GF 2 ), nystose (GF 3 ), and inulooligosaccharides (IOS). This study suggested the use of dahlia tuber extract and asparagus root powder as suitable substrates for inulinase production by the newly isolated Penicillium sp.

NFCC 2768, and its application in the generation of fructose and IOS.

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Provenance · 1 source records, 12 field assertions
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DataCite10.6084/m9.figshare.c.3247639.v112 d agoJSON v1
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