Table · dataset · 2025
Microbial Observatory (ISS-MO): Molecular characterization of Bacillus issensis, sp. nov., isolated from various quarters of the International Space Station
Listed in NASA Data Portal
As part of an ongoing effort to catalogue microbial communities inhabiting the International Space Station (ISS) crew-associated environmental samples were collected from the Japanese Kibo Russian and US research modules.
Description
Initial analysis based on 16S rRNA gene sequencing identified 11 Bacillus isolates (two from Kibo Japanese Experiment Module (JEM) four from US Segment Harmony Node 2 and five from Russian Segment Zvezda module sites) all belonging to the Bacillus anthracis-B. cereus-B. thuringiensis group.
Isolates were further characterized by whole genome comparative analysis. Each isolate was sequenced assembled and aligned against all members of the B. cereus sensu lato group. Based on genome size estimates (5.2 - 5.3 Mbp) strain nucleotide identity (>99.99%) and maximum likelihood phylogenetic placement all isolates were found to exhibit a very high level of similarity.
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With respect to gene content all isolates were inspected for cry proteins common to B. thuringiensis as well as toxins specific to B. cereus. No significant hits were found to any known cry genes while full-length matches were found for multiple B. cereus toxin genes. Finally the isolates were closely screened against B. anthracis genomes and no B. anthracis signatures were identified.
Notably all isolates contained the plcR ancestral C allele and lacked significant hits to pXO1 and pXO2 plasmids and toxins. The collective results from the analysis confirm that these strains belong to a previously uncharacterized Bacillus species hereafter referred to as Bacillus issensis. The type strain is ISSFR-003T (=NRRL B-65389T= DSMZ 101676T).
Links
Get the data
- Resource lsda.jsc.nasa.gov/scripts/experiment/exper.aspx?exp_index=13629 ↗
download · from data nasa gov
- Resource lsda.jsc.nasa.gov/scripts/mission/miss.aspx?mis_index=355 ↗
download · from data nasa gov
- Resource osdr.nasa.gov/bio/repo/data/studies/OSD-64 ↗
download · from data nasa gov
Where it is published
- NASA Data Portal dataset page data.nasa.gov/dataset/microbial-observatory-iss-mo-molecular-characterizatio… ↗
landing page · from data nasa gov
- DOI doi.org/10.26030/hk50-2z37 ↗
DOI / persistent id · from data nasa gov
Catalogue records · 1
- CKAN API data.nasa.gov/api/3/action/package_show?id=cc3bf1f9-1f09-480d-b29f-fe18ad413… ↗
metadata API · from data nasa gov
Topics
- From keywords
- Astronomy & Astrophysics · Earth & Environmental Science · Life Sciences · Ocean & Atmospheric Science · Physics
- Inferred from text
- Microbial taxonomy 76% · Sequencing 75%
Provenance · 1 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NASA Data Portal | cc3bf1f9-1f09-480d-b29f-fe18ad413204 | 8 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].anzsrc:field:310409 | enrichment · data nasa gov | taxonomy-embedding@1.0.0 | title+keywords+description (76%) |
| concepts[field].local:field:astronomy | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[field].local:field:ocean-atmospheric | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[field].local:field:physics | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · data nasa gov | keyword-concept-rules@1.0.0 | title+description (75%) |
| created_date | source · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| description | source · data nasa gov | connector:data_nasa_gov@1.0.0 | /notes |
| license_text | source · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| publication_date | source · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| title | source · data nasa gov | connector:data_nasa_gov@1.0.0 | /title |
| updated_date | source · data nasa gov | connector:data_nasa_gov@1.0.0 |