[BATS HPLC pigments] - HPLC and fluorometric derived phytoplankton pigment concentrations from seawater collected at the Bermuda Atlantic Time-series Study (BATS) site from October 1988 through June 2025 (Bermuda Atlantic Time-series Study)

Samples are typically collected from 12 depths in the upper 250 meters of the water column where by 4l of seawater is filtered under low vacuum through a 25mm GF/F filter. The filter is then flash frozen in liquid nitrogen and stored at -80C.

Shoreside, analysis is performed on a HPLC using a modified version of Wright et al., 1991 (modified by Dr R. Bidigare,University of Hawaii) which identifies the following pigments: Chlorophyll C3, Chlorophyll C2, Peridinin, 19'Butanoyloxyfucoxanthin, Fucoxanthin, 19'Hexanoyloxyfucoxanthin, Prasinoxanthin, Violoxanthin, Diadinoxanthin, Alloxanthin, Diatoxanthin, Lutein, Zeaxanthin, Chlorophyll b, Chlorophyll a, Divinyl, chlorophyll_a, Alpha and beta carotene.