Exploring the mechanism of Jiawei Simiao Powder on monosodium urate crystal-induced RAW264.7 cell inflammation via miR-146a regulation of the TLR4/NF-κB signaling pathway

The correlation between miR-146a and TLR4, MyD88, TRAF6, NF - κ B p65, and p-NF - κ B p65 was analyzed. As a result, compared with the control group, the expression of miR-16a in the model group cells was significantly reduced (P<0.05), while the mRNA and protein expression of TLR4, MyD88, TRAF6, p-NF - κ B p65 protein expression, and the expression levels of IL-1, IL-6, and TNF - α were significantly increased (P<0.05); Compared with the model group, the expression of miR-16a in the Jiawei Simiao San group and the colchicine group was significantly increased (P<0.05), while the mRNA and protein expression of TLR4, MyD88, TRAF6, and p-NF - κ B were significantly decreased (P<0.05), and the contents of IL-1, IL-6, and TNF - α were significantly decreased (P<0.05).

The correlation analysis results indicate that miR-146a is negatively correlated with TLR4, MyD88, TRAF6 mRNA and protein expression, as well as p-NF - κ B p65 protein expression (P<0.05). Conclusion: Jiawei Simiao San can alleviate MSU induced gout inflammation by upregulating miR-146a, inhibiting the activation of TLR4/NF - κ B signaling pathway, and reducing the expression of inflammatory factors.