rIL-37-mediated TMZ resistance in U251 cells

After TMZ exposure with or without rIL-37 treatment with 3 biological replicates set for each treatment group, the total RNA of U251 cells was extracted using TRIzol reagent, followed by DNase I treatment and purity verification. Poly(A) mRNAs were isolated and enriched from total RNAs using the NEBNext Poly(A) mRNA Magnetic Isolation Module (cat. no. E7490S; Illumina, Inc.), and cDNA libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit (cat. no. E7775S; Illumina, Inc.).

RNA seq was performed using an HiSeq 4000 platform (Illumina, Inc.). Differentially expressed genes (DEGs) were identified with a threshold set at a false discovery rate (FDR) of <0.05 and fold-change (FC) of >2 (|log2FC|>1), followed by functional enrichment analysis of DEGs using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG).