Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - Western blots of SDS-PAGE gels with primary antibodies against Hsp70p and Pgk1p

To answer this question, we utilised the disparate functions of the Hsp70's Ssa1 and 2 and their paralog Ssa4 in a yeast strain that lacks Ssa1/2 but has an ectopically increased production of Ssa4. We have gathered data on the behaviour of several different markers for protein aggregation under different circumstances, as well as data on proteins from other classes of molecular chaperones.

The bulk of the data is in the form of multichannel microscopy images from widefield microscopy, with a few sets of western blots of protein extracts. WT ssa12DD and ssa12DDSSA4OE: Western blots of SDS-PAGE gels with protein extracts from yeast in mid-exponential growth. The yeast was grown in complete synthetic media with 2 % glucose as carbon source.