Table · dataset · 2026
Proteomics-Guided Discovery of Candidate Enzymes for Degradation of a Rare Pyruvylated Glycosaminogalactan by a Newly Isolated Luteolibacter Strain
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Bacterial exopolysaccharides (EPS) display extensive structural diversity arising from variation in monosaccharide composition, glycosidic linkages, and non-carbohydrate modifications.
Description
However, the enzymes responsible for degrading many EPS structures remain unknown, reflecting the limited coverage of current carbohydrate-active enzyme (CAZyme) families. Here, we investigated the microbial degradation of EPS<sub><i>Lp</i></sub>, a high-molecular-mass glycosaminogalactan from Lactiplantibacillus pentosus KW1 containing the uncommon GalNAcβ1 → 2(4,6-<i>O</i>-<i>R</i>-pyruvyl)Gal motif.
Enrichment cultivation with EPS<sub><i>Lp</i></sub> as the main carbon source led to the isolation of <i>Luteolibacter</i> sp. VK1, a novel strain capable of utilizing this biopolymer. Size-exclusion chromatography and MALDI-TOF mass spectrometry showed that VK1 depolymerizes EPS<sub><i>Lp</i></sub> into defined oligosaccharides, consistent with endo-acting cleavage followed by further processing.
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By combining genomics, AlphaFold-based structural annotation and comparative proteomics, we identified candidate CAZymes potentially involved in EPS<sub><i>Lp</i></sub> catabolism. Notably, a secreted GH123 protein and a distantly related GH123-like protein were strongly associated with EPS<sub><i>Lp</i></sub>-dependent growth, suggesting that this enzyme family, or related structural homologues, may contribute to extracellular EPS<sub><i>Lp</i></sub> depolymerization.
Together, these findings support a candidate pathway for degradation of a structurally complex, pyruvylated bacterial glycan and highlight potentially novel EPS-active enzymes whose proposed roles, predicted through bioinformatic analyses, await experimental validation.
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Where it is published
- DOI doi.org/10.1021/acs.jproteome.6c00586.s002 ↗
DOI / persistent id · from figshare com
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from figshare com
Topics
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- Mass spectrometry 75%
Provenance · 5 source records, 19 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub · removed upstream | oai:figshare.com:article/34025007 | 14 d ago | JSON v1 |
| HKU DataHub · removed upstream | oai:figshare.com:article/34025007 | 13 d ago | JSON v1 |
| figshare | oai:figshare.com:article/34025007 | 13 d ago | JSON v1 |
| Loughborough Research Repository · removed upstream | oai:figshare.com:article/34025007 | 12 d ago | JSON v1 |
| UP Research Data Repository · removed upstream | oai:figshare.com:article/34025007 | 12 d ago | JSON v1 |
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|---|---|---|---|
| access_level | source · figshare com | connector:figshare_com@1.0.0 | |
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| concepts[field].anzsrc:group:3107 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
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| concepts[field].local:field:chemistry | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:computer-science-ai | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:economics-finance | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:engineering | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:humanities | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:ocean-atmospheric | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:social-science | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[modality].local:modality:mass-spectrometry | enrichment · figshare com | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/description |
| license | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/rights |
| publication_date | source · figshare com | connector:figshare_com@1.0.0 | |
| title | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/title |