Structure · dataset · 2026
<p>18S ONT tagged primers. Primers amplifying 1263 bp of the 18S rRNA gene (shown in purple) were modified with 8mer barcodes (represented in blue) for identifying the three replicates a, b, and c for each site. The Oxford Nanopore Technologies sequencing tags (depicted in red) were added from the PCR Barcoding Kit (SQK-BK004) according to their Four-Primer PCR protocol.</p>
Listed in figshare
<p>18S ONT tagged primers.
Description
Primers amplifying 1263 bp of the 18S rRNA gene (shown in purple) were modified with 8mer barcodes (represented in blue) for identifying the three replicates a, b, and c for each site. The Oxford Nanopore Technologies sequencing tags (depicted in red) were added from the PCR Barcoding Kit (SQK-BK004) according to their Four-Primer PCR protocol.</p>
Links
Where it is published
- DOI doi.org/10.1371/journal.pwat.0000557.t001 ↗
DOI / persistent id · from figshare com
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from figshare com
Topics
- From keywords
- Chemistry · Earth & Environmental Science · Ecology · Inorganic chemistry · Life Sciences · Microbiology
- Inferred from text
- Sequencing 75%
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| figshare | oai:figshare.com:article/34512922 | 6 h ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].anzsrc:group:3103 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Ecology'] |
| concepts[field].anzsrc:group:3107 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].anzsrc:group:3402 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Inorganic Chemistry'] |
| concepts[field].local:field:chemistry | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · figshare com | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/description |
| license | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/rights |
| publication_date | source · figshare com | connector:figshare_com@1.0.0 | |
| title | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/title |