Table · dataset · 2025
Single-molecule long-read methylation profiling reveals regional DNA methylation regulated by Elongator Complex Subunit 2 in Arabidopsis roots experiencing spaceflight
Listed in NASA Data Portal
Description
The Advanced Plant Experiment-04 - Epigenetic Expression (APEX-04-EpEx) experiment onboard the International Space Station examined the spaceflight-altered cytosine methylation in two genetic lines of Arabidopsis thaliana, wild-type Col-0 and the mutant elp2-5, which is deficient in an epigenetic regulator Elongator Complex Subunit 2 (ELP2). Whole-genome bisulfite sequencing (WGBS) revealed distinct spaceflight associated methylation differences, presenting the need to explore specific space-altered methylation at single-molecule resolution to associate specific changes over large regions of spaceflight related genes.
To date, tools of multiplexed targeted DNA methylation sequencing remain limited for plant genomes. This data set includes single-molecule profiling in user-defined targets using Flap-Enabled Next-Generation Capture (FENGC) on Arabidopsis root tissues to reveal precise modification of DNA methylation regulated by Elongator Complex Subunit 2 during spaceflight.
Links
Get the data
- Resource ncbi.nlm.nih.gov/bioproject/PRJNA857037 ↗
download · from data nasa gov
- Resource osdr.nasa.gov/bio/repo/data/studies/OSD-625 ↗
download · from data nasa gov
- Resource osdr.nasa.gov/bio/repo/data/missions/SpaceX-10 ↗
download · from data nasa gov
- Resource purl.bioontology.org/ontology/NCBITAXON/3702 ↗
download · from data nasa gov
Where it is published
- NASA Data Portal dataset page data.nasa.gov/dataset/single-molecule-long-read-methylation-profiling-reveal… ↗
landing page · from data nasa gov
- DOI doi.org/10.26030/rp5n-0d70 ↗
DOI / persistent id · from data nasa gov
Catalogue records · 1
- CKAN API data.nasa.gov/api/3/action/package_show?id=bba84c88-4122-44f2-b146-5b949a1c5… ↗
metadata API · from data nasa gov
Topics
- From keywords
- Astronomy & Astrophysics · Earth & Environmental Science · Life Sciences · Ocean & Atmospheric Science · Physics
- Inferred from text
- DNA methylation profiling 65% · Genetics 73% · Sequencing 75%
Provenance · 1 source records, 14 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NASA Data Portal | bba84c88-4122-44f2-b146-5b949a1c530f | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].anzsrc:group:3105 | enrichment · data nasa gov | taxonomy-embedding@1.0.0 | title+keywords+description (73%) |
| concepts[field].local:field:astronomy | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[field].local:field:ocean-atmospheric | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[field].local:field:physics | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[method].local:method:dna-methylation-profiling | enrichment · data nasa gov | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:sequencing | enrichment · data nasa gov | keyword-concept-rules@1.0.0 | title+description (75%) |
| created_date | source · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| description | source · data nasa gov | connector:data_nasa_gov@1.0.0 | /notes |
| license_text | source · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| publication_date | source · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| title | source · data nasa gov | connector:data_nasa_gov@1.0.0 | /title |
| updated_date | source · data nasa gov | connector:data_nasa_gov@1.0.0 |