Transcriptome sequencing dataset of the Pardosa pseudoannulata under cadmium and polystyrene microplastic stress

Total RNA extraction was performed with TRNzol Universal Reagent (TIANGEN Biotech, Beijing, China) according to the manufacturer’s instructions. Sequencing libraries were constructed using the Fast RNA-seq Lib Prep Kit V2 (Cat.

No. Rk20306, ABclonal, Wuhan, China). High-throughput sequencing was conducted for all samples on an Illumina NovaSeq X Plus instrument. Raw sequencing reads were processed to obtain clean reads by removing adapter sequences, reads containing poly-N regions, and low-quality reads.