Table · dataset · 2025
Long-read sequencing reveals increased occurrence of genomic variants and adenosine methylation in Bacillus pumilus SAFR-032 after long-duration flight exposure onboard the International Space Station
Listed in NASA Data Portal
Bacillus pumilus SAFR-032, an endospore-forming bacterial strain, was investigated to determine its methylation pattern (methylome) change, compared to ground control, after direct exposure to space conditions onboard the International Space Station (ISS) for 1.5 years.
Description
The resulting ISS-flown and non-flown strains were sequenced using the Nanopore MinION and an in-house method and pipeline to identify methylated positions in the genome.
Our analysis indicated genomic variants and m6A methylation increased in the ISS-flown SAFR-032. To complement the broader omics investigation and explore phenotypic changes, ISS-flown and non-flown strains were compared in a series of laboratory-based chamber experiments using an X-ray irradiation source (doses applied at 250, 500, 750, 1000 and 1250 Gy); results show a potentially higher survival fraction of ISS-flown DS2 at the two highest exposures.
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Taken together, results from this study document lasting changes to the genome by methylation, potentially triggered by conditions in spaceflight, with functional consequences for the resistance of bacteria to stressors expected on long-duration missions beyond low Earth orbit.
Links
Get the data
- Resource purl.bioontology.org/ontology/NCBITAXON/1408 ↗
download · from data nasa gov
- Resource osdr.nasa.gov/bio/repo/data/studies/OSD-383 ↗
download · from data nasa gov
Where it is published
- NASA Data Portal dataset page data.nasa.gov/dataset/long-read-sequencing-reveals-increased-occurrence-of-g… ↗
landing page · from data nasa gov
- DOI doi.org/10.26030/dk3z-b805 ↗
DOI / persistent id · from data nasa gov
Catalogue records · 1
- CKAN API data.nasa.gov/api/3/action/package_show?id=2bef6ea2-13c9-44b1-a6d2-245cce51e… ↗
metadata API · from data nasa gov
Topics
- From keywords
- Astronomy & Astrophysics · Earth & Environmental Science · Life Sciences · Ocean & Atmospheric Science · Physics
- Inferred from text
- Evolutionary biology 70% · Sequencing 75%
Provenance · 1 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NASA Data Portal | 2bef6ea2-13c9-44b1-a6d2-245cce51e0f5 | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].anzsrc:group:3104 | enrichment · data nasa gov | taxonomy-embedding@1.0.0 | title+keywords+description (70%) |
| concepts[field].local:field:astronomy | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[field].local:field:ocean-atmospheric | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[field].local:field:physics | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · data nasa gov | keyword-concept-rules@1.0.0 | title+description (75%) |
| created_date | source · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| description | source · data nasa gov | connector:data_nasa_gov@1.0.0 | /notes |
| license_text | source · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| publication_date | source · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| title | source · data nasa gov | connector:data_nasa_gov@1.0.0 | /title |
| updated_date | source · data nasa gov | connector:data_nasa_gov@1.0.0 |