Total and hydrocarbon-degrading bacteria in the permeable reactive barrier at Casey

More information about the dataset is presented on the summary worksheet of the download file - this information is copied below: Most probable number counts were carried out on material sampled from the permeable reactive barrier at Casey in Jan/Feb 2007. 4-tube MPN with three replicates for each sample. Most probable number was calculated using the MPN Calculator available from: http:/members.ync.net/mcuriale/mpn/index.html The barcode numbers are barcodes recorded in the Human Impacts Sample Tracking Database.

The sample name has the multiport location and depth of sample in m (eg MP01 0.45 is multiport 1 at 0.45m depth). Total heterotrophs were estimated using half-strength tryptone-soya broth in 96-well titre trays. 1:10 serial dilutions were made in a total of 200 microlitres of medium. Plates were incubated at 10°C for 7 days.

Plates were scored manually with visually turbid cells being positive. SAB-degrading bacteria were estimated using Bushnell-Haas broth and 5ul of SAB as carbon source. 1:10 serial dilutions were made in a total of 200 microlitres of medium. Plates were incubated at 10 degrees C for 14 days.

At this time 40 microlitres of INT solution were added and incubated for another two days (INT = 2.25 g/l of iodonitrotetrazolium chloride). Plates were scored manually, with the presence of a red precipitate or red colour being positive.