Replication Data for: A Design of Experiments Approach to Production of Medicinal Bioactives in Mitragyna speciosa (Korth.) Havil

Kratom products are growing in popularity for increasing energy, improving mood and alleviating minor pain, but availability of commercial plant material is limited in North America. Propagation relies primarily on shoot cuttings rooted in soils with high levels of humus and is relatively inefficient.

We used a Design of Experiments (DOE) approach to minimize the number of treatments necessary to optimize in vitro culture media to induce shoot and root morphogenesis in kratom explants. A clean mother stock of in vitro cultures was established and evaluated for medicinal bioactives via targeted and untargeted analyses by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). We found no significant difference in the quantified levels of mitragynine, melatonin, and some related metabolites, between in vitro grown and greenhouse grown plants.