Transcriptome profiling reveals the molecular mechanism of cuticular wax (bloom) formation during blueberry fruit development.

However, the transcriptional regulatory networks underlying its formation during fruit maturation remain largely unknown. In this study, we performed comparative transcriptome sequencing (RNA-seq) on blueberry fruits at three critical developmental stages: the early green fruit stage (stage 1), the color-break stage (stage 2), and the mature stage (stage 3).

A total of 9 cDNA libraries (three independent biological replicates per developmental stage) were constructed and sequenced. This dataset captures the dynamic transcriptional reprogramming during fruit development, specifically highlighting the spatiotemporal expression patterns of genes involved in lipid metabolism, very-long-chain fatty acid (VLCFA) elongation, triterpenoid biosynthesis, and ABC transporter-mediated secretion.