Data · dataset · 2025
Transcriptome sequencing of rheumatoid arthritis patient-derived synovial fibroblasts with or without NRP2 knockdown
Listed in ScienceDB
Synovial fibroblasts were isolated from synovial tissues of rheumatoid arthritis patients, passaged, and purified, and identified by flow cytometry.
Description
Cells from passages 3 to 8 were used for experiments. After transfection of siCon or siNRP2 via electroporation for 24 hours, the cells were stimulated with TNF for 2 or 20 hours, collected, and subjected to transcriptome sequencing.
RNA-seq and data analysis were performed by Seqhealth Technology Co., Ltd. (Wuhan, China). Total RNA was extracted using TRIzol, and 2 μg was used to prepare stranded mRNA libraries with the KC-Digital™ Kit (Illumina-compatible) per manufacturer protocols.
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Raw data were quality-filtered (Trimmomatic v0.36) and adaptor-trimmed, followed by in-house deduplication to remove PCR/sequencing biases. Exon-mapped reads were quantified (featureCounts, Subread-1.5.1), RPKM-normalized, and analyzed for differential expression (edgeR v3.12.1; p<0.05, |FC|≥2).
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.24014 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Medicine & Health · Social Science
- Inferred from text
- RNA sequencing 65% · Sequencing 75%
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.24014 | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:rna-seq | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license_text | source · scidb cn | connector:scidb_cn@1.0.0 | |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |