Isolation and identification of an antimicrobial peptide with high anticancer activity and selectivity, QUB-2584 from the skin secretion of Litoria caerulea

Their various and complex structural classification, physical-chemical properties, and mechanism contributing to their bioactivities have been discovered and well understood. Here, we focus on a bioactive peptide named QUB-2584 derived from the skin secretion of Litoria caerulea, which is an Australian frog.

Molecular cloning was used to confirm its sequence. Then the peptide was chemically synthesised by Solid Phase Peptide Synthesis, purified by RP-HPLC, and then analysed by MALDI-TOF MS to identify the peptide. Finally, QUB-2584 was tested by biological assays, it has shown significant antimicrobial activities against S. aureus, E. coli, and C. albicans with MIC at 2, 8, and 32 μM, respectively.