Escherichia coli CRISPR-Cas9 sgRNA On-target Activity Dataset (Wild-type, eSpCas9, and recA-Knockout Backgrounds)

The data were mainly derived from the construction of a prokaryotic sgRNA cleavage-activity prediction model and related supplementary experiments conducted at the College of Science, Dalian Maritime University, from December 2025 to September 2026.

The dataset integrates three prokaryotic benchmark datasets—wild-type Cas9 and its high-fidelity variants eSpCas9 and knoRecA_Cas9—sourced from an E. coli genome-scale sgRNA library (Wang et al., Nucleic Acids Research, 2018), comprising more than 137,000 sgRNA sequences of 43 nt together with their cleavage activities measured by high-throughput sequencing, and balanced binary labels (high-/low-efficiency) generated using the median editing efficiency of each dataset as the threshold.