Data · collection · 2013
X-ray diffraction data using methylmalonate-semialdehyde dehydrogenase from Oceanimonas doudoroffii
Listed in NASA Earthdata CMR
We successfully expressed and crystallized the dddC gene (Uniprot code G5CZI2) product from the Gram-negative marine bacterium Oceanimonas doudoroffii.
Description
The DddC is a methylmalonate-semialdehyde dehydrogenase (OdMMSDH) enzyme that is involved in dimethyl sulfonio propionate (DMSP) catabolism. DMSP is produced by marine phytoplankton and macroalgae, and is enzymatically metabolized into dimethylsulfide (DMS) or 3-methiolpropionate.
DMS is a major source of sulfur gases in marine environments and induces cloud nuclei condensation. As a result of DMSP catabolism, DMS plays an important role in the global sulfur cycle and climate change. In order to understand the structural details of OdMMSDH, the recombinant protein was over-expressed in Escherichia coli and successfully crystallized in 21% (w/v) PEG 3350 and 0.2 M potassium sodium tartrate, pH 7.5.
Read the rest (2 more)
Furthermore, a complete native diffraction data set was collected up to 2.9 Å resolution and processed in the P21212 space group with unit-cell parameters a = 156.7, b = 160.3, and c = 238.9 Å. Phase information was obtained by molecular replacement, and structure refinement and model building are in progress. To better understand the enzymatic mechanisms of MMSDH from O. doudoroffii (OdMMSDH), we have performed biochemical and structural studies.
As a first step towards its structural characterization, we here report the over-expression, purification, crystallization, and preliminary X-ray diffraction analysis of OdMMSDH.
Links
Documentation and papers
- PROJECT HOME PAGE kpdc.kopri.re.kr ↗
project · from NASA CMR
Catalogue records · 2
- CMR record cmr.earthdata.nasa.gov/search/concepts/C2244295350-AMD_KOPRI.html ↗
catalogue entry · from NASA CMR
- CMR UMM-JSON cmr.earthdata.nasa.gov/search/concepts/C2244295350-AMD_KOPRI.umm_json ↗
metadata API · from NASA CMR
Topics
- Stated by source
- Cloud Condensation Nuclei · Dimethyl Sulfide · NOT APPLICABLE · NOT APPLICABLE
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NASA Earthdata CMR | C2244295350-AMD_KOPRI | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| concepts[instrument].gcmd_instrument:not-applicable | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| concepts[measured_variable].gcmd:earth-science/atmosphere/aerosols/cloud-condensation-nuclei | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| concepts[measured_variable].gcmd:earth-science/atmosphere/atmospheric-chemistry/sulfur-compounds/dimethyl-sulfide | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| concepts[platform].gcmd_platform:not-applicable | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| created_date | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| description | source · NASA CMR | connector:nasa_cmr@1.0.0 | /umm/Abstract |
| publication_date | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| title | source · NASA CMR | connector:nasa_cmr@1.0.0 | /umm/EntryTitle |
| updated_date | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| version_label | source · NASA CMR | connector:nasa_cmr@1.0.0 |