Supplementary file 1_Short term ex vivo graft conditioning with venetoclax, fluticasone propionate, and IL-2 reduces graft versus host disease in mouse allogeneic hematopoietic cell transplantation.docx

GVHD is a major cause of post-transplant mortality, inspiring the development of prophylactic strategies to reduce GVHD severity. However, these involve administration of cytotoxic or immunosuppressive compounds into patients, increasing adverse side effects.

To address this issue, we have developed a strategy to reduce GVHD through ex vivo graft conditioning. Previously we demonstrated that pretreatment of mouse grafts with a single compound, fluticasone propionate, dramatically reduced GVHD symptoms after allogeneic transplantation. Here, we condition grafts in a combination of fluticasone propionate, venetoclax, and IL-2 for 6 hours ex vivo prior to transplantation.